FIVE MINUTES IN L.I.T.

Posted in Science by sallybr

L.I.T., you ask? Well, that stands for Laboratory of Iron Transport, and it’s where I spend most of my waking hours. In brief, we study how bacteria such as Escherichia coli and Listeria monocytogenes get iron from the environment and “swallow it up.” The metal is indispensable for bacteria as well as all other living organisms to survive, but it is very tricky to obtain. Iron can be compared to money in the sense that everyone who has it tries to protect it from being taken away. However, bacteria developed sophisticated systems to do just that: steal the iron from you and use it to survive. Since all pathogenic bacteria need to obtain iron to cause disease, we hope that our research will lead to the discovery of new weapons to fight infections.

We thought – actually, let me give full credit to Phil for that – he thought it would be fun to walk through the lab and ask what each person was doing, in an informal and fun way. Unfortunately, two of our undergrad students were not available when the video was made: Noah Long and Dallas Hyder. It was tricky enough to get everyone else there at the same time. I think the video turned out pretty good, as it was totally improvised.

Check it out here.

It makes me very sad to see Brittany Nairn, our super star Research Professor, getting ready to leave our group. She is the person who shows up in the video once Phil goes through a closed-door to our darkened “Fluorescence Spectroscopy Room.” We wish she could stay longer as a member of our team, but unfortunately that’s not possible. She will go on to write her own story, and we know she will be successful and happy no matter the path life takes her. Our whole lab will miss her terribly…

CREDITS IN ORDER OF APPEARANCE

Yan Shipelskiy – Graduate student working super hard to develop a full system of evaluation of iron uptake in Gram-positive bacteria

Olivia Eliasson – Undergrad student working with Dr. Nairn to evaluate compounds with anti-bacterial activity linked to blocking of iron uptake

Dr. Somnath Chakravorty – Post-doc who recently joined our lab, working on iron uptake genes from Klebsiella pneumoniae

Aritri Majumdar – Graduate student performing very tricky experiments to evaluate the effect of bacterial membrane integrity on iron uptake

Ashish Somvanshi – Graduate student who just joined our lab, also working on iron uptake by Klebsiella pneumoniae

Dr. Brittany Nairn – Research Professor who joined our lab in August last year, and developed from scratch a whole system to evaluate iron uptake in a pathogen called Acinetobacter baumannii

Dr. Sally Newton – yours truly, molecular biologist by day, food blogger by night. Performing her favorite type of experiment using radioactive iron. Because, you know… some like it hot!

Director, Producer and Narrator, Dr. Phillip Klebba… My favorite scientist in the whole wide world!

Our undergrad student Noah Long, missing from the video, but proudly showing the results of his labor: the best batch of enterobactin ever purified in our lab! How cool is that look?

😉

Before I leave, I invite you to watch this video of Dr. Phil Klebba explaining in more detail the research of our group. Sorry, ladies, he is already taken.

6May2016

PECAN CRUSTED CHICKEN FROM SOUTHERN AT HEART

Posted in Poultry by sallybr

Some recipes sound quite tasty but disappoint when you make them. Not this one. Tasted as good as it sounded… Funny thing is, I wasn’t sure it would be a winner because chicken breasts can be a bit tricky when baked. However, the meat was tender and moist, it looked pretty nice when sliced, and I heard “this is really good” several times during our Sunday dinner. It is a bit involved to make on weeknights, but I guess one could spread the preparation, assembling the “roulades” the evening before and keeping them in the fridge without the pecan coating. Next evening, all that’s left to do is coat the meat with crumbs and bake. The recipe comes from Damaris Phillips show on FoodTV, Southern at Heart. I find her delightful, upbeat and funny. Even though for the most part her style of cooking is a bit too heavy for my taste, every once in a while I find something that calls my name. Like this chicken. Flavorful and quite elegant to boot.

PECAN CRUSTED STUFFED CHICKEN BREASTS
(adapted from Damaris Phillips)

Four boneless, skinless chicken breasts
salt and ground black pepper
4 ounces fresh goat cheese, crumbled
1 tablespoon chopped fresh dill
2 teaspoons grated orange zest
2 eggs
2/3 cup dried breadcrumbs
2/3 cup ground pecans
Coconut oil spray

Heat the oven to 400 degrees F.

Place the chicken breasts between two pieces of plastic wrap and pound to 1/4-inch thick. Pat the chicken dry and sprinkle liberally with salt and pepper on both sides. Sprinkle the goat cheese lengthwise on one half of each breast; then sprinkle with dill, and orange zest. Fold in the short ends as if folding a Mexican burrito, then, starting on the half with cheese, roll up into a tight cylinder. Close the seams with toothpicks or tie with kitchen twine.

Whisk the eggs in a wide, shallow dish with 1 tablespoon water. In a separate dish, combine the breadcrumbs and ground pecans. Sprinkle the stuffed chicken with salt and pepper. Dip in the egg mixture and then in the breadcrumb mixture; shake off excess breading.

Place the breaded chicken on a wire rack set over a baking sheet and spray with coconut oil. Bake to an internal temperature of 160 degrees F, about 25 minutes. Remove from the oven and let rest for 5 minutes. Remove the toothpicks. Cut in slices and serve.

ENJOY!

to print the recipe, click here

Comments: I know that goat cheese and dill are a match made in heaven, but you know what made this dish a real winner? The orange zest. In fact, the sweet citric flavor was even more intense two days later when we had leftovers for dinner. I served it with butternut squash “noodles” and asparagus, but of course the chicken would go well with many different side dishes. For instance, Damaris paired it with a Southern risotto, very hearty.

This recipe would be great for a dinner party, as you can prepare it all in advance and place the meat in the oven half an hour before you want to sit down for dinner. Keep in mind not to over-process the pecans, so that you get a bit more crunch on the topping. In typical Sally mode, I totally forgot that I have some pecan flour in the freezer. I bet it would be great to use maybe 50-50 with the bread crumbs, intensifying the pecan flavor. Something to consider for next time…

ONE YEAR AGO: Lamb Shanks en Papillote with Cauliflower-Celeriac Purée

TWO YEARS AGO: Chestnut Brownies and a Blog Award!

THREE YEARS AGO: Quinoa with Cider-Glazed Carrots

FOUR YEARS AGO: Celebrate Wednesday: Heirloom Tomatoes Steal the Show

FIVE YEARS AGO: Pain de Provence

SIX YEARS AGO: Golspie Loaf, from the Scottish Highlands

1May2016

SECRET RECIPE CLUB: CHOC-ORANGE MINI CAKES & A BONUS RECIPE

Posted in Desserts, Desserts, Sweets, Secret Recipe Club, Veggies by sallybr

(Reprinted with permission from Adrienne Hedger)

And here we are landing firmly in the month of May. This year is flying by, if you ask me… First Monday of the month means fun. It is Reveal Day for The Secret Recipe Club, that event in which bloggers are paired in secret and cook a recipe from their assigned site. This month I got a food blog that was new to me: Chit Chat Chomp, hosted by Leigh, who blogs from Melbourne, a place I would love to visit one day. Leigh’s site is a thing of beauty, very stylish, elegant, amazing photos, and great prose. Basically, a must-read food blog! She summarizes her approach to cooking in a delightful way:

Recipes inspired by my travels through France, adapted for everyday simplicity and filled to the brim with nutrition. Mostly gluten and refined sugar-free, my recipes lean towards food that inspires, heals and nourishes, but above all else, it’s fresh, organic and simple.

Recipes inspired by my travels through France… She’s got my full attention! In fact, she describes herself as a lover of all things French. You can imagine the smile I opened when I read that line, right? I spent quite a bit of time stalking her blog, but jumped on a recipe right away for a reason I will classify as “The Swedish Effect.” Small parenthesis is needed. We have an undergraduate student from Sweden in our lab called Olivia. She happened to mention that April 15th was her “name day” back home. I was puzzled, but then learned that in Sweden each day of the year is associated with a particular name, as you can see in this site. April 15th is the name day for Olivia (girls) and Oliver (boys). Olivia said that her Grandma always gave her a nice card on that day to celebrate the occasion. So, I thought it would be cool to bake something starting with the letter O and bring to the lab on that day. Orange-Chocolate mini-cakes seemed perfect! Obviously, I jumped on the recipe with no hesitation whatsoever. But, my decision had a small problem associated with it, which led me to make a second recipe for today’s reveal. Read on…

ORANGE-CHOC MINI CAKES
(from Chit Chat Chomp)

Makes 6 mini-cakes

1 orange
1 cup almond meal
1 tsp baking powder
2 large eggs
1 Tbsp raw cacao powder
1 Tbsp rice malt syrup or raw honey
handful of raw cacao nibs
handful of sunflower seeds (I omitted for lack of sunflower seeds)

Heat oven to 320 F and line a mini cupcake pan with 8 cupcake papers

Place the orange in a pot and cover with water. Bring to the boil then reduce the heat to low and simmer for 1 hour. Allow to cool.

Chop the cooked orange into chunks and place in a blender and blitz until smooth. Add all other ingredients (except cacao nibs and sunflower seeds) to the blender and pulse until combined.

Divide the batter between your cupcake papers, filling close to the top as they will not rise by much, and top with the cacao nibs and sunflower seeds. Pop into the oven for approximately 30 to 40 minutes, or until a skewer comes out clean when inserted in the middle.

ENJOY!

to print the mini-cake recipe, click here

Comments: As Leigh mentioned, the only tricky part of this recipe is to boil the orange. Tricky in the sense that you need to dedicate one hour for that step. Essentially hands-free, though. The smell in the kitchen as the orange simmers…. wonderful! Once the orange is ready and cooled down, the batter will be ready in minutes. Now, let’s talk about the “small problem.” The recipe made six cupcakes, enough for each of our lab members to enjoy, but Phil and I could not have any. In short, I made a recipe for The Secret Recipe Club, but never got to taste it. I had only one way to restore my dignity as a blogger: choose a second recipe from Chit Chat Chomp, and make it too… and that’s what I did, so today you get two recipes instead of one. How’s that for problem solving?

Without further ado… my bonus recipe from Leigh’s great blog:

VEGGIE BREAD
(from Chit Chat Chomp)

1 + 1/2 cup almond meal
3/4 cups arrowroot flour
1/2 teaspoon sea salt
1/2 teaspoon baking soda
5 eggs
1 + 1/2 teaspoon apple cider vinegar
1 large zucchini, grated
1 large carrot, grated
1/2 cup finely grated Parmesan cheese
2 tablespoons pepitas.

Heat the oven to 325 F and line a standard loaf pan with parchment paper.

In a large bowl, mix together the almond meal, arrowroot, salt and baking soda. In a separate bowl whisk the eggs lightly with the apple cider vinegar. Add zucchini, carrot and cheese and mix well.

Add the egg mix to the dry ingredients. Mix to combine. Pour the dough into the prepared loaf tin and sprinkle with pepitas.

Bake for 35-40 minutes or until the top starts turning golden and a skewer comes out clean. Remove from the oven and transfer to a wire rack. Allow to cool for five minutes before cutting into slices.

ENJOY!

to print the Veggie Bread recipe, click here

Comments: We loved this bread! It is obviously gluten-free, no regular flour, but the texture reminded me of quick breads, except that this had considerable more moisture. Not too dense like some gluten-free breads turn out. Leigh said it freezes well, but we did not get to test that, in three days it was gone. I enjoyed a slice at lunchtime, and was surprised that Phil did that too, he prefers his bread to be loaded with gluten. This one had so much flavor that the lack of wheat flour was not a big deal. Once it sits in the fridge for a while, the best way to bring it back to life is toasting it… paired with juicy tomatoes, it was out of this world delicious!

I wanted to try to make croutons out of it. Not sautéed because I am afraid they would crumble, it is a very delicate bread. But maybe spraying the croutons with coconut or olive oil then toasting them in the oven. I bet they would turn out amazing on a kicked-up version of Caesar salad, or over a massaged kale concoction. Definitely a bread to play with.

I must say it was not easy to decide on which recipe to make as the bonus. I really wanted to make her Savoury Muffins, not only because the recipe enticed me, but for the write-up about it. You gotta go and read it… it has to do with France… And, if the weather was a little more appropriate, I would love a bowl of her luscious Carrot Soup… Another heavy contender was the Zucchini Noodle with Kale Pesto, which is pretty much my favorite type of meal these days.

Leigh, I loved getting your blog as my assignment this month, and feel sorry I could not taste those cute mini-cupcakes… Thinking back, I should have made a double batch, but I only thought about it after they were all baked and I had no time to boil another orange and start over. It was getting close to bedtime then… oh, well. Such is life!

Please make sure to click on the blue frog sitting patiently at the end of this post, so you can marvel at the posts that my virtual friends from Group A made for their assignments.

Adrienne, thank you again for allowing me to share your cartoon on the blog!
I love your work!

ONE YEAR AGO: In My Kitchen, May 2015

TWO YEARS AGO: P90X3, a Review of Tony Horton’s Latest Fitness Program

THREE YEARS AGO: Pasta and Mussels in Saffron Broth

FOUR YEARS AGO: Triple Chocolate Brownies

FIVE YEARS AGO: Shanghai Soup Dumplings

SIX YEARS AGO: Bite-sized Chocolate Pleasure